Dexamethasone Inhibits High Glucose–, TNF- –, and IL-1 –Induced Secretion of Inflammatory and Angiogenic Mediators from Retinal Microvascular Pericytes

METHODS. HRMP and HREC phenotypes were verified by growth factor stimulation of intracellular calcium–ion mobilization. Glucocorticoid receptor phosphorylation was assessed with an anti-phospho-Ser glucocorticoid receptor antibody. Secretion of 89 inflammatory and angiogenic proteins were compared in cells incubated with (1) normal (5 mM) or high (25 mM) D-glucose and (2) control medium, TNF(10 ng/mL), or IL-1 (10 ng/mL), with or without dexamethasone (1 nM to 1 M). The proteins were compared by using multianalyte profile testing.